Nucleotides for cycle sequencing of gc-rich templates

The present invention relates to a mixture comprising 7-deaza dGTP or a functional analogue thereof and dITP. The invention further relates to a method for sequencing and/or amplification of a nucleic acid sequence comprising the steps of preparing a sequencing mixture comprising, among others, 7-de...

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Hauptverfasser: VOSS, HARTMUT, MOTZ, MICHAEL
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creator VOSS, HARTMUT
MOTZ, MICHAEL
description The present invention relates to a mixture comprising 7-deaza dGTP or a functional analogue thereof and dITP. The invention further relates to a method for sequencing and/or amplification of a nucleic acid sequence comprising the steps of preparing a sequencing mixture comprising, among others, 7-deaza dGTP or a functional analogue thereof and dITP, further (NH4)2SO4, a nucleic acid sequence to be sequenced and/or amplified, and some enzymatic activity for incorporating dNTPs and/or ddNTPs or derivatives thereof.
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language eng ; fre ; ger
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
title Nucleotides for cycle sequencing of gc-rich templates
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