Nucleotides for cycle sequencing of gc-rich templates

The present invention relates to a mixture comprising 7-deaza dGTP or a functional analogue thereof and dITP. The invention further relates to a method for sequencing and/or amplification of a nucleic acid sequence comprising the steps of preparing a sequencing mixture comprising, among others, 7-de...

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Bibliographische Detailangaben
Hauptverfasser:	VOSS, HARTMUT, MOTZ, MICHAEL
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a mixture comprising 7-deaza dGTP or a functional analogue thereof and dITP. The invention further relates to a method for sequencing and/or amplification of a nucleic acid sequence comprising the steps of preparing a sequencing mixture comprising, among others, 7-deaza dGTP or a functional analogue thereof and dITP, further (NH4)2SO4, a nucleic acid sequence to be sequenced and/or amplified, and some enzymatic activity for incorporating dNTPs and/or ddNTPs or derivatives thereof.