Method for quantification of an analyte

The invention provides a new method for the quantification of an analyte, wherein in a first step, the analyte is amplified by an amplifying agent. Subsequently, the amount of amplification product is determined as a function of time and the extremae of first, second or n th order derivatives are ca...

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Bibliographische Detailangaben
Hauptverfasser: WITTWER, CARL T., DR, LOHMANN, SABINE, GUTEKUNST, MARTIN
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:The invention provides a new method for the quantification of an analyte, wherein in a first step, the analyte is amplified by an amplifying agent. Subsequently, the amount of amplification product is determined as a function of time and the extremae of first, second or n th order derivatives are calculated. The obtained values can then be used to calculate the initial concentration of the analyte.