YEAST CELLS COMPRISING AT LEAST TWO COPIES OF A DESIRED GENE INTEGRATED INTO THE CHROMOSOMAL GENOME AT MORE THAN ONE NON-RIBOSOMAL RNA ENCODING DOMAIN, PARTICULARLY WITH KLUYVEROMYCES

YEAST CELLS COMPRISING AT LEAST TWO COPIES OF A DESIRED GENE INTEGRATED INTO THE CHROMOSOMAL GENOME AT MORE THAN ONE NON-RIBOSOMAL RNA ENCODING DOMAIN, PARTICULARLY WITH KLUYVEROMYCES

The present invention provides for a yeast cell comprising at least two copies of a desired gene integrated into its chromosomal genome, wherein said genome comprises at least two DNA domains suitable for integration of one or more copies of said desired gene, which domains share substantial sequenc...

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Bibliographische Detailangaben
Hauptverfasser: NOORDERMEER-VAN DER HAAK, ADRIANA, CORNELIA, MARIA, VAN OOIJEN, ALBERT, JOHANNES, JOSEPH, SWINKELS, BART, WILLEM
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention provides for a yeast cell comprising at least two copies of a desired gene integrated into its chromosomal genome, wherein said genome comprises at least two DNA domains suitable for integration of one or more copies of said desired gene, which domains share substantial sequence homology and are non-ribosomal RNA encoding DNA domains, and wherein at least two of said substantially homologous non-ribosomal RNA encoding domains DNA domains have at least one copy of the said desired gene integrated. The invention also provides methods for making yeast cells according to the invention, as well as the use thereof for making a protein, a peptide or metabolite.