Integrally attached and operable multiple reaction vessels

A reaction vessel for confined amplification and detection of nucleic acid material. The vessel features a plurality of adjacent chambers, each chamber comprising a front wall, a back wall, two side walls, and a bottom wall, the front and back walls terminating in an upper opening at a top edge of said front and back walls, a side wall of each chamber comprising a side wall in common with an adjacent chamber so as to integrally connect the chambers side-by-side; the front wall of each chamber including a liquid access port spanning all of the chambers below the top edge, the common side walls terminating at the port; and a movable elastomeric plug mounted within the upper opening above the port, shaped to block the port of each of the chambers and to stopper each the chamber when moved below the top edge, the plug spanning across all of the chambers in the vessel so as to close off the port simultaneously for all of the chambers when moved below the top edge.