Use of an alcohol-salt buffer wash for the efficient recovery of mycobacteria and mycobacterial DNA from respiratory sediment

The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of .7 to .9 g/ml and a boiling point greater than 50 DEG C. The invention also relates to methods fo...

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Bibliographische Detailangaben
Hauptverfasser:	RASHMI, MEHTA, BACKUS, JOHN, W, KERSCHNER, JOANNE H, KREADER, CAROL
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of .7 to .9 g/ml and a boiling point greater than 50 DEG C. The invention also relates to methods for concentrating bacteria and free bacterial nucleic acids from a biological sample that involve mixing with the sample a density-lowering agent and a monovalent salt.