PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF -i(RHODOTORULA GRACILIS) IN HOST CELLS
Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula grac...
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| creator | MORENO VALLE, MIGUEL ANGEL DIEZ GARCIA, BRUNO SALTO MALDONADO, FRANCISCO VITALLER ALBA, ALEJANDRO COLLADOS DE LA VIEJA, ALFONSO BARREDO FUENTE, JOSE LUIS GARCIA LOPEZ, JOSE LUIS CORTES RUBIO, ESTRELLA ALONSO PALACIOS, JORGE SCHLEISSNER SANCHEZ, CARMEN |
| description | Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula gracillis producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of Rhodotorula gracillis with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme. |
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The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula gracillis producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of Rhodotorula gracillis with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme.</description><edition>6</edition><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1998</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19980520&DB=EPODOC&CC=EP&NR=0843015A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19980520&DB=EPODOC&CC=EP&NR=0843015A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MORENO VALLE, MIGUEL ANGEL</creatorcontrib><creatorcontrib>DIEZ GARCIA, BRUNO</creatorcontrib><creatorcontrib>SALTO MALDONADO, FRANCISCO</creatorcontrib><creatorcontrib>VITALLER ALBA, ALEJANDRO</creatorcontrib><creatorcontrib>COLLADOS DE LA VIEJA, ALFONSO</creatorcontrib><creatorcontrib>BARREDO FUENTE, JOSE LUIS</creatorcontrib><creatorcontrib>GARCIA LOPEZ, JOSE LUIS</creatorcontrib><creatorcontrib>CORTES RUBIO, ESTRELLA</creatorcontrib><creatorcontrib>ALONSO PALACIOS, JORGE</creatorcontrib><creatorcontrib>SCHLEISSNER SANCHEZ, CARMEN</creatorcontrib><title>PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF -i(RHODOTORULA GRACILIS) IN HOST CELLS</title><description>Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula gracillis producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of Rhodotorula gracillis with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1998</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwjAUQNEuDqL-wxt1CLRUwTUkL00gzStJCtYlFIkgiBbq_2MHP8Dp3uGsi9R5EhgCKPKwvOyFcQ1EjYDuOrQIkvHWOOLCSAK6DJIHBFLAHnuvSVIk31sOjV-ENeEAxoGmEEGgtWFbrO7jc867XzcFKIxCszy9U56n8ZZf-ZOwK8_HuqxOvKr_IF_bRzKJ</recordid><startdate>19980520</startdate><enddate>19980520</enddate><creator>MORENO VALLE, MIGUEL ANGEL</creator><creator>DIEZ GARCIA, BRUNO</creator><creator>SALTO MALDONADO, FRANCISCO</creator><creator>VITALLER ALBA, ALEJANDRO</creator><creator>COLLADOS DE LA VIEJA, ALFONSO</creator><creator>BARREDO FUENTE, JOSE LUIS</creator><creator>GARCIA LOPEZ, JOSE LUIS</creator><creator>CORTES RUBIO, ESTRELLA</creator><creator>ALONSO PALACIOS, JORGE</creator><creator>SCHLEISSNER SANCHEZ, CARMEN</creator><scope>EVB</scope></search><sort><creationdate>19980520</creationdate><title>PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF -i(RHODOTORULA GRACILIS) IN HOST CELLS</title><author>MORENO VALLE, MIGUEL ANGEL ; DIEZ GARCIA, BRUNO ; SALTO MALDONADO, FRANCISCO ; VITALLER ALBA, ALEJANDRO ; COLLADOS DE LA VIEJA, ALFONSO ; BARREDO FUENTE, JOSE LUIS ; GARCIA LOPEZ, JOSE LUIS ; CORTES RUBIO, ESTRELLA ; ALONSO PALACIOS, JORGE ; SCHLEISSNER SANCHEZ, CARMEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP0843015A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>1998</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MORENO VALLE, MIGUEL ANGEL</creatorcontrib><creatorcontrib>DIEZ GARCIA, BRUNO</creatorcontrib><creatorcontrib>SALTO MALDONADO, FRANCISCO</creatorcontrib><creatorcontrib>VITALLER ALBA, ALEJANDRO</creatorcontrib><creatorcontrib>COLLADOS DE LA VIEJA, ALFONSO</creatorcontrib><creatorcontrib>BARREDO FUENTE, JOSE LUIS</creatorcontrib><creatorcontrib>GARCIA LOPEZ, JOSE LUIS</creatorcontrib><creatorcontrib>CORTES RUBIO, ESTRELLA</creatorcontrib><creatorcontrib>ALONSO PALACIOS, JORGE</creatorcontrib><creatorcontrib>SCHLEISSNER SANCHEZ, CARMEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MORENO VALLE, MIGUEL ANGEL</au><au>DIEZ GARCIA, BRUNO</au><au>SALTO MALDONADO, FRANCISCO</au><au>VITALLER ALBA, ALEJANDRO</au><au>COLLADOS DE LA VIEJA, ALFONSO</au><au>BARREDO FUENTE, JOSE LUIS</au><au>GARCIA LOPEZ, JOSE LUIS</au><au>CORTES RUBIO, ESTRELLA</au><au>ALONSO PALACIOS, JORGE</au><au>SCHLEISSNER SANCHEZ, CARMEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF -i(RHODOTORULA GRACILIS) IN HOST CELLS</title><date>1998-05-20</date><risdate>1998</risdate><abstract>Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula gracillis producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of Rhodotorula gracillis with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; ger |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF -i(RHODOTORULA GRACILIS) IN HOST CELLS |
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