PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF -i(RHODOTORULA GRACILIS) IN HOST CELLS

PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF -i(RHODOTORULA GRACILIS) IN HOST CELLS

Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula grac...

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Hauptverfasser: MORENO VALLE, MIGUEL ANGEL, DIEZ GARCIA, BRUNO, SALTO MALDONADO, FRANCISCO, VITALLER ALBA, ALEJANDRO, COLLADOS DE LA VIEJA, ALFONSO, BARREDO FUENTE, JOSE LUIS, GARCIA LOPEZ, JOSE LUIS, CORTES RUBIO, ESTRELLA, ALONSO PALACIOS, JORGE, SCHLEISSNER SANCHEZ, CARMEN
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula gracillis producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of Rhodotorula gracillis with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme.