NOVEL 1,2-DIOXETANE COMPOUNDS WITH HALOALKOXY GROUPS, METHODS OF PREPARATION AND USE

A chemiluminescent assay method and compositions are described which use a haloalkoxy group-substituted dioxetane which is deprotected by a hydrolytic enzyme to undergo a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with a haloalkoxy group whi...

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Bibliographische Detailangaben
Hauptverfasser: EICKHOLT, ROBERT, ARGHAVANI, ZAHRA, RASHID, KHALEDUR, AKHAVAN-TAFTI, HASHEM
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:A chemiluminescent assay method and compositions are described which use a haloalkoxy group-substituted dioxetane which is deprotected by a hydrolytic enzyme to undergo a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with a haloalkoxy group which can be triggered by a reagent to generate light are disclosed. Haloalkoxy group-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.