IMPROVEMENT OF AN EXPRESSION VECTOR FOR PRODUCTION OF RECOMBINANT PROTEINS

The invention relates to a method of enhancing the transcription of a gene in a DNA construct incorporated into the genome of a eukaryotic host cell, said DNA construct comprising a structural gene for a desired protein or polypeptide and a gene promoter upstream of the structural gene, said method...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	SLIVA, DANIEL, LOBIE, PETER, HALDOSEN, LARS-ARNE, NORSTEDT, GUNNAR, ENBERG, BERTIL, WOOD, TIM
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method of enhancing the transcription of a gene in a DNA construct incorporated into the genome of a eukaryotic host cell, said DNA construct comprising a structural gene for a desired protein or polypeptide and a gene promoter upstream of the structural gene, said method comprising the steps of: (i) providing fused upstream of said promoter at least one Spi 2.1 GH response element, or a part thereof comprising the nucleotide sequence TTCTGAGAA, and (ii) exposing the said DNA construct to signals evoked by a lactogenic pituitary hormone.