DNA encoding thermostable pyrophosphatase

A DNA that encodes a thermostable pyrophosphatase (PPase) is provided. The DNA is obtained using a DNA probe consisting of SEQ ID NO: 1. Also provided are vectors comprising said DNA and host cells transformed with such a vector. The invention also provides methods for producing thermostable pyropho...

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Bibliographische Detailangaben
Hauptverfasser:	WANG, ALICE MING, GELFAND, DAVID HARROW
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A DNA that encodes a thermostable pyrophosphatase (PPase) is provided. The DNA is obtained using a DNA probe consisting of SEQ ID NO: 1. Also provided are vectors comprising said DNA and host cells transformed with such a vector. The invention also provides methods for producing thermostable pyrophosphatase, and methods for isolating a target DNA fragment comprising a DNA coding for a thermostable pyrophosphatase from a bacterium of the genus Thermus. The thermostable pyrophosphatase prepared in accordance with the present invention is preferably packed in a kit and can be used in a nucleic acid amplification method.