Improved methodology for colorimetrically determining the concentration of white blood cells in a biological fluid

A composition, method and test device for determining the presence of leukocytes, esterase or protease in a test sample are disclosed. The composition comprises an ester which is hydrolyzed in the presence of leukocyte, esterase or protease to form a reaction product which couples with a diazonium s...

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Bibliographische Detailangaben
Hauptverfasser:	NOFFSINGER, JAMES, SMITH, MELVIN D, PUGIA, MICHAEL J
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A composition, method and test device for determining the presence of leukocytes, esterase or protease in a test sample are disclosed. The composition comprises an ester which is hydrolyzed in the presence of leukocyte, esterase or protease to form a reaction product which couples with a diazonium salt to produce a detectable color change. The composition further comprises the salt of an alkaline earth metal which stabilizes the composition during manufacture and prevents the occurrence of false or background color change due to reactivity of the diazonium salt in the absence of leukocyte, esterase or protease.