Fast DNA assay for the detection of chinolon resistant staphylococcus aureus in clinical material

In der Erfindung werden Starteroligonukleotide (Primer), DNA-Sonden und Testverfahren zum schnellen Nachweis von chinolonresistenten Staphylococcus aureus Erregern in klinischem Probenmaterial beschrieben. Primers for amplification and specific detection of point mutations in Gyrase A are new: (i) f...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ENDERMANN, RAINER, BREMM, KLAUS-DIETER, SPRINGER, WOLFGANG
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	In der Erfindung werden Starteroligonukleotide (Primer), DNA-Sonden und Testverfahren zum schnellen Nachweis von chinolonresistenten Staphylococcus aureus Erregern in klinischem Probenmaterial beschrieben. Primers for amplification and specific detection of point mutations in Gyrase A are new: (i) for detecting C/T mutation at position 2533: (1) and (4); (ii) for detecting T/G mutation at position 2532: (2) and (4); and (iii) for detecting G/A mutation at position 2544: (3) and (4). ATCACCCTCATGGTGACTT (1); ATCACCCTCATGGTGACE (2); ATGGTGACTCATCTATTTATA (3); GTCCGCCTCCACGTTCTT (4) Also new are: (A) oligonucleotide probe (5), opt. labelled, that hybridises with DNA or RNA of quinolone-resistant Staphylococci: GTACGTATGGCTCAAGATTTCAGTTATCGTTATCCG (5); and (B) a polynucleotide probe (6; sequence of 534 bp reproduced in the specification), or its fragments, opt. labelled, that hybridises as (5).