Method for the enrichment of cells modified by ballistic transfer

A process is claimed for introducing substances into cells by ballistic transfer using accelerated micro-projectiles, which have the substance adsorbed on them or otherwise durably or transiently bound to them or which are formed from the substance or a soln. of the substance, so that the micro-proj...

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Bibliographische Detailangaben
Hauptverfasser:	SCHROFF, MATTHIAS, WITTIG, BURGHARDT, DR
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A process is claimed for introducing substances into cells by ballistic transfer using accelerated micro-projectiles, which have the substance adsorbed on them or otherwise durably or transiently bound to them or which are formed from the substance or a soln. of the substance, so that the micro-projectiles penetrate into the cells without killing them. The process is characterised in that ferromagnetic particles are also introduced into the cells by ballistic transfer. The cells are then placed in a magnetic field. The cells, that are not magnetically retained, are removed from the magnetic field by washing and the retained cells are collected.