Immunoassay elements comprising polymers containing vanadium IV (V (+4)) ions

A dry immunoassay analytical element, for assaying a ligand, comprising in the following order: (a) a layer containing a labeled ligand; (b) a spreading layer ; (c) a receptor layer containing a fixed concentration of an immobilized receptor for the labeled ligand and the receptor is i) covalently b...

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Bibliographische Detailangaben
Hauptverfasser: MESSING, CALVIN R, DANIELSON, SUSAN J, PONTICELLO, IGNAZIO S, HILBORN, DAVID A, DANIEL, DANIEL S
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:A dry immunoassay analytical element, for assaying a ligand, comprising in the following order: (a) a layer containing a labeled ligand; (b) a spreading layer ; (c) a receptor layer containing a fixed concentration of an immobilized receptor for the labeled ligand and the receptor is i) covalently bonded to polymeric beads having a diameter in the range of 0.1 to 5 mu m and ii) dispersed in a polymeric binder and (d) a support; characterized in that the spreading layer contains a water soluble polymer containing vanadium IV (V ions.