Dextranase enzyme, method for its production and DNA encoding the enzyme

A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungu...

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Hauptverfasser:	DELGADO BOADA, JULIO MARCOS, GONZALEZ MARTINEZ, MARIA ELENA, MARGOLLEZ CLARK, EMILIO, CAMPANA, HERNAN ROCA, CREMATA ALVAREZ, JOSE ALBERTO, GARCIA GARCIA, BIANCA MAR A, CURBELO, DANIA MATEU, JIMENEZ, EFRAIN RODRIGUEZ, MORERA CORDOVA, VIVIAN, RAICES PEREZ-CASTANEDA, MANUEL RAFAEL, HERRERA MARTINEZ, LUIS S, PATRON, CARLOS FERNANDEZ, GARCIA FERNANDEZ, ROSSANA
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.