LEUKINFERON PREPARATION

Une nouvelle composition de cytokine, le procédé pour sa préparation et l'utilisation de la composition dans l'immunothérapie de différentes maladies telles que les infections bactériennes, les infections virales et les cancers, sont décrits. Le nouveau procédé comprend l'addition de deux nouveaux ingrédients dénommés transal et d'un complexe d'inhibiteurs de protéinase à une culture de leucocytes induite par voie virale. La composition de cytokine résultante comprend des interférons de leucocytes humains, l'Interleuken 1, un facteur de nécrose tumorale, un facteur d'inhibition de migration et un facteur d'inhibition de migration de leucocytes dans leurs proportions naturelles. PCT No. PCT/CA93/00381 Sec. 371 Date Aug. 23, 1994 Sec. 102(e) Date Aug. 23, 1994 PCT Filed Sep. 20, 1993 PCT Pub. No. WO94/06820 PCT Pub. Date Mar. 31, 1994A cytokine composition is prepared for use in immunotherapy of various diseases. The composition is prepared by culturing leucocytes previously infected with a virus in a culture medium containing a transal composition isolated from plasma and a complex of proteinase inhibitors composition isolated from plasma, separating leucocytes from the medium, removing impurities from the medium, and recovering a cytokine composition containing the transal composition and the complex of protease inhibitors composition. The transal composition contains transferrin, albumin and a 40 kDa protein and the complex of protease inhibitors composition contains alpha 2-macroglobulin, a 160 kDa protein, an 80-60 kDa protein and a 20 kDa protein. The transal composition is prepared by mixing plasma with chloroform and recovering an aqueous phase, precipitating gamma globulins from the aqueous phase with polyethylene glycol to recover another aqueous phase, passing this aqueous phase through an anion exchange column and recovering proteins that bind to the column. The complex of protease inhibitors composition is prepared using steps for preparing the transal composition except that additional steps are used of collecting eluate from the anion exchange column, passing the eluate through a Cu++ chelate anion exchange column and recovering proteins that bind to the column.