A method of using eukaryotic expression vectors comprising the BK virus enhancer
A method for producing a protein that is naturally gamma carboxylated, properly folded, and processed and wherein the protein is encoded in a recombinant DNA vector such that the protein is expressed when a eukaryotic host cell containing the vector is cultured under suitable expression conditions,...
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Format: | Patent |
Sprache: | eng ; fre ; ger |
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Zusammenfassung: | A method for producing a protein that is naturally gamma carboxylated, properly folded, and processed and wherein the protein is encoded in a recombinant DNA vector such that the protein is expressed when a eukaryotic host cell containing the vector is cultured under suitable expression conditions, which comprises:(a) inserting the vector into an adenovirus-transformed, human embryonic kidney cell; and(b) culturing said host cell of step a) under growth conditions and in media containing sufficient vitamin K for carboxylation. |
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