Colorimetric assay for the determination of density or activity of viable cells

A convenient and rapid assay for activity and density of living cells in many samples is provided. That is, a oxidized type quinone such as 1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone or tetramethyl-p-benzoquinone is reacted with a system to be determined. The Hydrogen peroxide thus produced is...

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Bibliographische Detailangaben
Hauptverfasser:	NISHIMOTO, FUKIKO, YAMASHOJI, SHIRO
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A convenient and rapid assay for activity and density of living cells in many samples is provided. That is, a oxidized type quinone such as 1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone or tetramethyl-p-benzoquinone is reacted with a system to be determined. The Hydrogen peroxide thus produced is indirectly reacted with the colorimetric reagent by using enzyme or directly with the colorimetric reagent, the intensity of the color development thus caused is determined with a spectro phtometer(a colorimeter). A proper enzyme of the acceptor of hydrogen peroxide is peroxidase, indirect color reagent is sodium salt of N-(carboxymethylaminocarbonyl)-4,4'-bis(dimethylamino)-diphenylamine or leucocrystal violet are suitable for indirect colometric reagent, and direct colorimetric reagent is cytochrome c peroxidase.