PRECIPITATION PROCESS FOR EXOCELLULAR PROTEINS
Exocellular proteins from microorganisms are sepd. from a filtered fermentation broth by (a) removing substances which hinder protein pptn., with a solid adsorbent, (b) concentrating the remaining soln. to 30-40 wt.% protein content, (c) allowing the proteins to ppte. at pH 6-10, and sepg., opt. wit...
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Zusammenfassung: | Exocellular proteins from microorganisms are sepd. from a filtered fermentation broth by (a) removing substances which hinder protein pptn., with a solid adsorbent, (b) concentrating the remaining soln. to 30-40 wt.% protein content, (c) allowing the proteins to ppte. at pH 6-10, and sepg., opt. with addn. of a precipitant for proteins to accelerate the pptn. |
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