IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS

Disclosed is a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog comprising...

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Bibliographische Detailangaben
Hauptverfasser:	BARTFELD, DANIEL, LIFSHITZ, RUTH, HADARY, DANY
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog comprising treating the composition to separate soluble proteins from whole cells, cellular debris and insoluble proteins to obtain a solution containing such soluble proteins, including the a, b and c isoforms; treating the resulting solution with soluble proteins to produce three separate solutions, each of which has an increased concentration of one of either of the a, b or c isoform; recovering the separate solution with an increased concentration of the b isoform; combining the separate solution with an increased concentration of the a isoform with the separate solution with an increased concentration of the c isoform; treating the resulting combined solution to produce a solution with an increased concentration of the b isoform and recovering the then-resulting solution with an increased concentration of the b isoform.