RELEASE AND ISOLATION OF N-GLYCANS AND O-GLYCANS

The invention relates to a method for the release of N- and O-glycans from a glycoconjugate and to isolation of such glycans. The invention provides a method which includes subjecting a glycoconjugate to the influence of a hydrazine reagent, said glycoconjugate being substantially salt-free and subs...

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Bibliographische Detailangaben
Hauptverfasser:	BRUCE, JAMES, PAREKH, RAJESH BHIKHU, MERRY, ANTHONY HUGH
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS THEREFOR CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS BASED THEREON DERIVATIVES THEREOF GENERAL METHODS OF ORGANIC CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY ORGANIC MACROMOLECULAR COMPOUNDS PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS POLYSACCHARIDES SUGARS TESTING THEIR PREPARATION OR CHEMICAL WORKING-UP TRANSPORTING
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Beschreibung
Zusammenfassung:	The invention relates to a method for the release of N- and O-glycans from a glycoconjugate and to isolation of such glycans. The invention provides a method which includes subjecting a glycoconjugate to the influence of a hydrazine reagent, said glycoconjugate being substantially salt-free and substantially anhydrous and said hydrazine reagent being substantially anhydrous, and controlling conditions under which the glycoconjugate is subjected to the influence of the hydrazine reagent so as to cause release of N- and O-glycans from the glycoconjugate in such a way as to allow subsequent recovery of N- and O-glycans in substantially unreduced and intact form. Released glycans may be isolated in accordance with the invention by use of a chromatographic material and, if required, performing an N-acetylation reaction and converting any acetohydrazone derivative to unreduced N-and O-glycans.