Methods and apparatus for incubation and sterilization

Apparatus for continuous incubation of e.g. animal cells, has sterilising filters (25,26,27,28) in the connecting lines (35,36) which transfer liquid incubation medium from a medium supply storage tank (21) to the incubator (22), and from the incubator to a medium extraction tank (23). The sterilising filters prevent the spread of contamination from one element of the apparatus to the next. Sterilisation of the apparatus e.g. by steam (31) need not include pipe portions (B-C; F-G) between the filters, since contamination in these portions is trapped by the filters. By not sterilising these pipe portions, control units (C1,C2,C3) of the three system elements can function independently of one another in sterilising operations.