NOVEL RESTRICTION ENDONUCLEASE

A new restriction enzyme, Mfe I, has been discovered. Mfe I recognizes the sequence CAATTG and cuts at the recognition sequence C'AATTG and generates compatible cohesive ends with EcoRI cleaved fragments. Various utilities of the enzyme have been described.

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Bibliographische Detailangaben
Hauptverfasser: LEONARD, WARREN, J, HALDEN, NANCY, F, WOLF, JULIE, B
Format: Patent
Sprache:eng
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Zusammenfassung:A new restriction enzyme, Mfe I, has been discovered. Mfe I recognizes the sequence CAATTG and cuts at the recognition sequence C'AATTG and generates compatible cohesive ends with EcoRI cleaved fragments. Various utilities of the enzyme have been described.