Diagnostic and amplification methods overcoming a primer-target mismatch at the primer 3'end

Methods for amplifying and detecting a predetermined target nucleic acid in a biological specimen are known, and can be accomplished even when there is a mismatch in a single position between a primer and the target nucleic acid. The mismatch is located at or near the 3 min end of the primer. Disclo...

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Bibliographische Detailangaben
Hauptverfasser:	FINDLAY, JOHN BRUCE, BERGMEYER, LYNN
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods for amplifying and detecting a predetermined target nucleic acid in a biological specimen are known, and can be accomplished even when there is a mismatch in a single position between a primer and the target nucleic acid. The mismatch is located at or near the 3 min end of the primer. Disclosed herein is a method for overcoming such a mismatch, and is particularly useful for detection of a nucleic acid sequence which is not fully known, or where there is considerable heterogeneity in DNA target from patient samples. The method is characterized by using a primer having a nucleotide with a thymine base at the position of the mismatch, the primer being used to prime the target and to form primer extension products.