Method for the synthesis of ribonucleic acid (RNA)

The invention relates to a method for the synthesis of ribonucleic acid starting from deoxyribonucleic acid in which a target sequence is present. Said DNA is treated with restriction enzyme(s) followed by strand separation. Subsequently one uses one nucleic acid primer containing a promoter sequenc...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: DAVEY, CHERYL, WIELAARD, FRITS, MALEK, LAWRENCE T, LENS, PETER FRANKLIN
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:The invention relates to a method for the synthesis of ribonucleic acid starting from deoxyribonucleic acid in which a target sequence is present. Said DNA is treated with restriction enzyme(s) followed by strand separation. Subsequently one uses one nucleic acid primer containing a promoter sequence coupled to a nucleotide sequence which corresponds to a part of the target sequence. The invention further relates to a test kit for synthesizing RNA.