Hybrid plasmid for producing subtilisin Carlsberg in bacillus

Hybrid plasmids for microorganisms of the genus Bacillus, containing a starting plasmid which is suitable for Bacillus and has one or more cleavage sites for restriction enzymes such as Ava I and, if required, a resistance marker and, inserted into the starting plasmid, an isolated double-stranded DNA containing: - a promoter which is recognised by the RNA polymerase of microorganisms of the genus Bacillus, - ribosome binding sites - start codon - structural gene which codes only for subtilisin Carlsberg or its fragments with proteolytic activity or variants with proteolytic activity including their leader sequences - stop codon it being possible for oligonucleotide chains with up to 400 base pairs without specific expression ability to be present calculated downstream from the stop codon, characterised in that the inserted double-stranded DNA contains promoter and structural gene of subtilisin Carlsberg from Bacillus licheniformis and starts with position -227 calculated from the start codon of the pro/pre-subtilisin.