Method for producing 20-deoxotylosin

The present invention, utilizing recombinant DNA technology, provides a novel method for obtaining 20-deoxotylosin from a tylosin producing microorganism. 20-deoxotylosin is useful as an antibiotic with a microbial inhibitory activity similar to tylosin. The method comprises culturing a tylosin-prod...

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Bibliographische Detailangaben
Hauptverfasser:	SENO, EUGENE THOMAS, COX, KAREN LEIGH
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention, utilizing recombinant DNA technology, provides a novel method for obtaining 20-deoxotylosin from a tylosin producing microorganism. 20-deoxotylosin is useful as an antibiotic with a microbial inhibitory activity similar to tylosin. The method comprises culturing a tylosin-producing strain of Streptomyces, said strain: (a) comprising one or more mutations that prevent the expression of tylosin biosynthetic genes tylI and R, in which R is one or more tylosin biosynthetic genes selected from tylA, tylB, tylL, tylM, tylH, tylD, tylJ, tylG, tylK, tylC, tylE, or tylF; and, (b) being transformed with a recombinant DNA cloning vector which comprises DNA which complements said mutation or mutatons in R thereby allowing for the expression of R; said culture conditions suitable for cell growth, expression of R and production of 20-deoxotylosin.