Gene amplification

Gene amplification

Improved means are provided for obtaining enhanced production of proteins encoded by structural genes of interest in a host based on transfecting the host with an expression vector comprising a wild-type amplifiable gene and the predetermined structural gene. The host is typically not complemented b...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: MORSER, MICHAEL J, CASHION, LINDA M, COLBY, WENDY W
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Improved means are provided for obtaining enhanced production of proteins encoded by structural genes of interest in a host based on transfecting the host with an expression vector comprising a wild-type amplifiable gene and the predetermined structural gene. The host is typically not complemented by the amplifiable gene product. If desired, another selection marker may be utilized to select a desired population of cells prior to the amplification.