HYBRIDIZATION METHOD AND REAGENT KIT THEREFOR

A hybridisation method of detecting a nucleic acid comprising: (a) filtering a solution in which the presence of target nucleic acid or copy of target nucleic acid molecule is to be detected to remove insoluble impurities from the solution (b) producing from the filtrate a conjugate of an insoluble...

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Bibliographische Detailangaben
Hauptverfasser:	KORPELA, KAI MIKAEL, RANKI, TUULA MARJUT, TENHUNEN, JUKKA TAPANI, LAAKSONEN, MATTI HEIKKI SAKARI, SYVANEN, ANNRISTINE ELIZABETH, JARVINEN, ANNE MARITTA, WECKMAN, ARJA MARJATTA, KARLSSON, STEFAN HENRIK, SODERLUND, HANS ERIK, BUCHERT, PETRI UOLEVI, LONNQVIST, GUNNEL SOLVEIG KRISTINA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A hybridisation method of detecting a nucleic acid comprising: (a) filtering a solution in which the presence of target nucleic acid or copy of target nucleic acid molecule is to be detected to remove insoluble impurities from the solution (b) producing from the filtrate a conjugate of an insoluble particle and hybridized target nucleic acid or hybridized copy of target nucleic acid molecule, the hybridized nucleic acid or copy molecule being attached to the particle via a nucleic acid probe or optionally, in the case of a hybridized copy molecule, via a primer by means of which the copy was made; (c) separating the conjugate from any excess probe and/or primer by filtration and washing; and (d) detecting the amount of conjugate present. c