Methods of regulating protein glycosylation

Methods for producing a heterologous protein or polypeptide are disclosed. A preferred method utilizes a fungal cell carrying a defect in a gene whose product is required for the addition of outer chain oligosaccharide moieties to glycoproteins, the cell transformed with a first DNA construct compri...

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Bibliographische Detailangaben
Hauptverfasser: WELCH, SUSAN K, MACKAY, VIVIAN L, YIP, CARLI L
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:Methods for producing a heterologous protein or polypeptide are disclosed. A preferred method utilizes a fungal cell carrying a defect in a gene whose product is required for the addition of outer chain oligosaccharide moieties to glycoproteins, the cell transformed with a first DNA construct comprising a regulated promoter followed downstream by a DNA sequence which complements the defect, and a second DNA construct comprising a second promoter followed downstream by a DNA sequence encoding a secretion signal and a DNA sequence encoding a heterologous protein or polypeptide. A yeast cell having a Mnn9 phenotype and capable of producing colonies of normal morphologies in the absence of osmotic stabilization is also disclosed.