B-Cell stimulating factor

BSF-1 was derived from malignant cells and purified by use of various techniques, including adsorption, ion exchange chromatography and reverse phase high performance liquid chromatography. By these techniques, the BSF-1 was purified to homogeneity. The high purification of the BSF-1 has made possib...

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Hauptverfasser:	CLEVENGER, WILLIAM RUSSELL, HOPP, THOMAS PATRICK, PRICE, VIRGINIA LEE, MARCH, CARL JACK, GILLIS, STEVEN, EISENMAN, JUNE R, MOCHIZUKI, DIANE YUKIKO, SHANEBECK, KURT DAVID, CONLON, PAUL JEROME, GRABSTEIN, KENNETH HOWARD
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	BSF-1 was derived from malignant cells and purified by use of various techniques, including adsorption, ion exchange chromatography and reverse phase high performance liquid chromatography. By these techniques, the BSF-1 was purified to homogeneity. The high purification of the BSF-1 has made possible the sequencing of the amino acid residues at the N-terminal portion of its protein molecules. From the amino acid sequencing information, a radiolabeled oligonucleotide probe corresponding to a portion of the amino acid sequence of the BSF-1 molecule was synthesized and then used to probe a cDNA library prepared from polyadenylated mRNA extracted from cell lines known to produce BSF-1. Through this procedure, a cDNA clone containing the BSF-1 gene was isolated, sequence and mature BSF-1 expressed. The isolated cDNA clone was then radiolabeled and used as a large probe for screening cDNA libraries of other species of animals for homologous BSF-1 clones.