5-Hydroxy-3-indoleacetic acid fluorescence polarization immunoassay

The present invention is directed to a fluorescence polarization immunoassay for determining the 5-hydroxy-3-indoleacetic acid content in body fluids, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immu...

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Hauptverfasser: ADAMCZYK, MACIEJ BOGDAN, BRASHEAR, ROY JEFFREY, FLENTGE, CHARLES ARTHUR
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:The present invention is directed to a fluorescence polarization immunoassay for determining the 5-hydroxy-3-indoleacetic acid content in body fluids, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them. The assay is conducted by measuring the degree of polarization of plane polarized light that has been passed through a sample containing anti-serum and tracer.