PULSED-FIELD GRADIENT GEL ELECTROPHORETIC APPARATUS

PULSED-FIELD GRADIENT GEL ELECTROPHORETIC APPARATUS

Electrophoretic separation apparatus (10) comprises a gel matrix medium (28) for supporting macromolecular particles to be separated, the medium (28) having opposed faces separated by a thickness of medium small in relation to the dimension of the faces and including one or more separation tracks (3...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: LAAS, WILLIAM A, GARDINER, KATHELEEN J, PATTERSON, DAVID
Format: Patent
Sprache:eng
Schlagworte:
APPARATUS THEREFOR
CHEMISTRY
ELECTROLYTIC OR ELECTROPHORETIC PROCESSES
ELECTROLYTIC OR ELECTROPHORETIC PROCESSES FOR THE PRODUCTIONOF COMPOUNDS OR NON-METALS
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
SEPARATION
TESTING
TRANSPORTING
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Beschreibung
Zusammenfassung:Electrophoretic separation apparatus (10) comprises a gel matrix medium (28) for supporting macromolecular particles to be separated, the medium (28) having opposed faces separated by a thickness of medium small in relation to the dimension of the faces and including one or more separation tracks (30); first electrode means (40,46) positioned about said medium for applying, upon energization, a first electric field (48) across the or each separation track, the direction of the first field intersecting both planar faces and oriented at an angle which is acute with respect to a first planar face; second electrode means (42,44) positioned about said medium for applying, upon energization, a second electric field (50) across the or each separation track, the direction of the second field intersecting both planar faces and oriented at an angle which is acute with respect to a second planar face; and means for alternately and repetitively energizing the first and second electrode means (62).