Process for recovering polypeptides localized in the periplasmic space of yeast

A process is disclosed for releasing into aqueous medium polypeptides produced by yeasts and localized at least partially within the periplasmic space thereof. The process consists in treating the yeasts with a system comprising (i) a neutral and water-soluble mineral salt, and (ii) a water-soluble...

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Bibliographische Detailangaben
Hauptverfasser: DELCOUR, JEAN M.A.G, DE BAETSELIER-VAN BROEKOVEN, ANNIE J.F, CRAHAY, JACQUES, HANOTIER, JACQUES D.V
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:A process is disclosed for releasing into aqueous medium polypeptides produced by yeasts and localized at least partially within the periplasmic space thereof. The process consists in treating the yeasts with a system comprising (i) a neutral and water-soluble mineral salt, and (ii) a water-soluble nonionic surfactant of the polyethoxylated alkylphenol type having an HLB comprised between 8 and 15.