A method for the generation and detection of enzyme variants with enhanced themostability and activity
A method for transforming thermophilic microorganisms. A method for transforming thermophilic microorganisms is disclosed comprising: (a) forming a protoplast from a thermophilic microorganism; (b) introducing a plasmid chimera into the protoplast; and (c) causing regeneration of the cell wall of th...
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| creator | GOLD, LAWRENCE M LIAO, HANS H MCDONELL, MICHAEL W HIRSH, DAVID I |
| description | A method for transforming thermophilic microorganisms. A method for transforming thermophilic microorganisms is disclosed comprising: (a) forming a protoplast from a thermophilic microorganism; (b) introducing a plasmid chimera into the protoplast; and (c) causing regeneration of the cell wall of the protoplast. A novel regeneration medium is employed in the transformation method. This method involves the use of novel plasmid chimera. The plasmid chimeras include a newly discovered thermostable promoter. A method of isolating promoters capable of functioning at elevated temperatures is included. A method for selecting thermostable variants of gene products of cloned genes in thermophilic microorganisms is also disclosed. |
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A method for transforming thermophilic microorganisms is disclosed comprising: (a) forming a protoplast from a thermophilic microorganism; (b) introducing a plasmid chimera into the protoplast; and (c) causing regeneration of the cell wall of the protoplast. A novel regeneration medium is employed in the transformation method. This method involves the use of novel plasmid chimera. The plasmid chimeras include a newly discovered thermostable promoter. A method of isolating promoters capable of functioning at elevated temperatures is included. A method for selecting thermostable variants of gene products of cloned genes in thermophilic microorganisms is also disclosed.</description><edition>4</edition><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1985</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19850424&DB=EPODOC&CC=EP&NR=0138075A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19850424&DB=EPODOC&CC=EP&NR=0138075A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GOLD, LAWRENCE M</creatorcontrib><creatorcontrib>LIAO, HANS H</creatorcontrib><creatorcontrib>MCDONELL, MICHAEL W</creatorcontrib><creatorcontrib>HIRSH, DAVID I</creatorcontrib><title>A method for the generation and detection of enzyme variants with enhanced themostability and activity</title><description>A method for transforming thermophilic microorganisms. A method for transforming thermophilic microorganisms is disclosed comprising: (a) forming a protoplast from a thermophilic microorganism; (b) introducing a plasmid chimera into the protoplast; and (c) causing regeneration of the cell wall of the protoplast. A novel regeneration medium is employed in the transformation method. This method involves the use of novel plasmid chimera. The plasmid chimeras include a newly discovered thermostable promoter. A method of isolating promoters capable of functioning at elevated temperatures is included. A method for selecting thermostable variants of gene products of cloned genes in thermophilic microorganisms is also disclosed.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1985</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjUEKwjAQRbNxIeod5gJCSxHdFqm4dOG-jMnEBJpJSYZKPb2xeABXn_f5j79WtoVA4qIBGxOII3gSU0LxkQHZgCEhvVC0QPyeA8GEySNLhpcXV0qHrMl87RCz4MMPXubFxqJOBbZqZXHItPvlRsGlu5-vexpjT3lEXV6l725V3Zyq46Gtmz8mH-4FP2Y</recordid><startdate>19850424</startdate><enddate>19850424</enddate><creator>GOLD, LAWRENCE M</creator><creator>LIAO, HANS H</creator><creator>MCDONELL, MICHAEL W</creator><creator>HIRSH, DAVID I</creator><scope>EVB</scope></search><sort><creationdate>19850424</creationdate><title>A method for the generation and detection of enzyme variants with enhanced themostability and activity</title><author>GOLD, LAWRENCE M ; LIAO, HANS H ; MCDONELL, MICHAEL W ; HIRSH, DAVID I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP0138075A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>1985</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>GOLD, LAWRENCE M</creatorcontrib><creatorcontrib>LIAO, HANS H</creatorcontrib><creatorcontrib>MCDONELL, MICHAEL W</creatorcontrib><creatorcontrib>HIRSH, DAVID I</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>GOLD, LAWRENCE M</au><au>LIAO, HANS H</au><au>MCDONELL, MICHAEL W</au><au>HIRSH, DAVID I</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>A method for the generation and detection of enzyme variants with enhanced themostability and activity</title><date>1985-04-24</date><risdate>1985</risdate><abstract>A method for transforming thermophilic microorganisms. A method for transforming thermophilic microorganisms is disclosed comprising: (a) forming a protoplast from a thermophilic microorganism; (b) introducing a plasmid chimera into the protoplast; and (c) causing regeneration of the cell wall of the protoplast. A novel regeneration medium is employed in the transformation method. This method involves the use of novel plasmid chimera. The plasmid chimeras include a newly discovered thermostable promoter. A method of isolating promoters capable of functioning at elevated temperatures is included. A method for selecting thermostable variants of gene products of cloned genes in thermophilic microorganisms is also disclosed.</abstract><edition>4</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | A method for the generation and detection of enzyme variants with enhanced themostability and activity |
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