A method for the generation and detection of enzyme variants with enhanced themostability and activity

A method for the generation and detection of enzyme variants with enhanced themostability and activity

A method for transforming thermophilic microorganisms. A method for transforming thermophilic microorganisms is disclosed comprising: (a) forming a protoplast from a thermophilic microorganism; (b) introducing a plasmid chimera into the protoplast; and (c) causing regeneration of the cell wall of th...

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Bibliographische Detailangaben
Hauptverfasser: GOLD, LAWRENCE M, LIAO, HANS H, MCDONELL, MICHAEL W, HIRSH, DAVID I
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A method for transforming thermophilic microorganisms. A method for transforming thermophilic microorganisms is disclosed comprising: (a) forming a protoplast from a thermophilic microorganism; (b) introducing a plasmid chimera into the protoplast; and (c) causing regeneration of the cell wall of the protoplast. A novel regeneration medium is employed in the transformation method. This method involves the use of novel plasmid chimera. The plasmid chimeras include a newly discovered thermostable promoter. A method of isolating promoters capable of functioning at elevated temperatures is included. A method for selecting thermostable variants of gene products of cloned genes in thermophilic microorganisms is also disclosed.