CLONING VECTORS

The present invention discloses selectable pairs of recombinant DNA cloning vectors for use in Streptomyces and related organisms. A pair of recombinant DNA cloning vectors comprises:a) plasmid pEL7, andb) a second plasmid that is functionally dependent upon plasmid pEL7, said second plasmid compris...

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Bibliographische Detailangaben
Hauptverfasser:	NAKATSUKASA, WALTER MITSUO, FAYERMAN, JEFFREY TOBIN, MABE, JAMES ALBERT
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention discloses selectable pairs of recombinant DNA cloning vectors for use in Streptomyces and related organisms. A pair of recombinant DNA cloning vectors comprises:a) plasmid pEL7, andb) a second plasmid that is functionally dependent upon plasmid pEL7, said second plasmid comprising a restriction fragment of plasmid pEL7 and one or more different DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive host cell, said host cell being susceptible to transformation, cell division, and culture.