A process for the production of a polypeptide

A process is described for the production of the proteolytic enzyme chymosin. The process involves cleavlng chymosin precursor polypeptides such as methionineprochymosin, preprochymosin and methionine-chymosin expressed from a host organism which has been transformed with a vector system carrying th...

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Hauptverfasser:	DOEL, MICHAEL TERENCE, HARRIS, TIMOTHY JOHN ROY, LOWE, PETER ANTHONY, CAREY, NORMAN HENRY, EMTAGE, JOHN SPENCER
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A process is described for the production of the proteolytic enzyme chymosin. The process involves cleavlng chymosin precursor polypeptides such as methionineprochymosin, preprochymosin and methionine-chymosin expressed from a host organism which has been transformed with a vector system carrying the relevant gene. The gene sequence of preprochymosin is given. The vector systems used are plasmids adapted for the transformation of either bacterial or eukaryotic host organisms. E. Coli and saccharomyces cerevisiae are specific examples of suitable host organisms. There is also described an oligonucleotide capable of being used as a specific hybridisation probe or transcription primer for mRNA containing the nucleotide sequence coding for chymosin. A microassay for chymosin activity is also described.