NUKLEINSYREMUTATIONSDETEKTERING UNDER ANVENDELSE AF MAGNETISK KUGLEAKTUERING OG DETEKTERING AF MAGNETISKE KUGLER

The present invention relates to a method for distinguishing mutations, single nucleotide polymorphisms or other variants in a target nucleic acid sequence from the wild-type sequence in a sample. The method comprises the steps of surface binding magnetic beads via a sandwich hybridization in which...

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Bibliographische Detailangaben
Hauptverfasser:	HIELTJES, Martinus Johannes, FEITSMA, Harma Martine, EVERS, Toon Hendrik, DE REGT, Bram, VAN ZON, Joannes Baptist Adrianus Dionisius
Format:	Patent
Sprache:	dan
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for distinguishing mutations, single nucleotide polymorphisms or other variants in a target nucleic acid sequence from the wild-type sequence in a sample. The method comprises the steps of surface binding magnetic beads via a sandwich hybridization in which a bead-bound probe hybridizes with one end of a target nucleic acid, and a surface-bound probe hybridizes with the other end of the same target nucleic acid, applying stringency on the hybridization by magnetic force and/or temperature, determining the amount of magnetic beads remaining attached to the surface, and correlating the amount of magnetic beads remaining attached to the surface with the presence and/or absence of mutations, SNP variants or other variants in the target nucleic acid.