FORBEDREDE FREMGANGSMÅDER TIL MODIFICERING AF MÅLNUKLEINSYRER

FORBEDREDE FREMGANGSMÅDER TIL MODIFICERING AF MÅLNUKLEINSYRER

The invention is directed to improved methods for modification of target nucleic acids in a cell, wherein a fusion nucleic acid (fuNA) molecule, comprising a guide nucleic acid (gNA) molecule covalently linked to at least one donor nucleic acid (doNA) molecule, and introducing said fuNA molecule int...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: MCELVER, John, A, DE WILDE, Chris, FELLE, Max, Fabian, NEUTEBOOM, Leendert, W, DE PINHO BARROCO, Rosa
Format: Patent
Sprache:dan
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention is directed to improved methods for modification of target nucleic acids in a cell, wherein a fusion nucleic acid (fuNA) molecule, comprising a guide nucleic acid (gNA) molecule covalently linked to at least one donor nucleic acid (doNA) molecule, and introducing said fuNA molecule into one or more cells comprising the target NA molecule, and introducing into one or more cells a site directed nucleic acid modifying polypeptide, e.g. Cas9. Homology arms in the donor nucleic acid which may be RNA, DNA or PNA, have at least 15 nucleotides 100 % complementary to 15 nucleotides in the target. Also claimed is a fuNA molecule which consists of RNA.