Fremgangsmåde til påvisning af hæmoplasma

A method for detecting at least one haemoplasma species within the group of haemoplasmas of the genus Mycoplasma in a biological sample, said method comprising the steps of: i) treating said sample to release bacterial nucleic acid in the sample, ii) amplifying a segment of a 16S rRNA gene by real-t...

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Bibliographische Detailangaben
Hauptverfasser:	Rosengarten, Renate, ZIMMERMAN, Carl-Ulrich Richard
Format:	Patent
Sprache:	dan
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for detecting at least one haemoplasma species within the group of haemoplasmas of the genus Mycoplasma in a biological sample, said method comprising the steps of: i) treating said sample to release bacterial nucleic acid in the sample, ii) amplifying a segment of a 16S rRNA gene by real-time PCR (qPCR) using an oligonucleotide primer pair, said oligonucleotide primer pair consisting of a forward primer comprising at least 10 consecutive bases of SEQ ID 1 and a reverse primer comprising at least 10 consecutive bases of SEQ ID 2, thereby producing an amplification product, and iii) detecting the amplification product by means producing a detection signal correlating with the amount of the amplification product which is indicative of any haemoplasma species within the genus Mycoplasma; and a composition comprising the forward primer and the reverse primer.