STABILE CREATINAMIDINOHYDROLASEMUTANTER

A creatine amidinohydrolase in which the alanine in position 109 of the amino-acid sequence of the wild-type enzyme is replaced by valine and, where appropriate, additionally one or more other amino acids of the wild-type enzyme are mutated is more stable than the wild-type enzyme and is therefore m...

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Bibliographische Detailangaben
Hauptverfasser:	SCHUMACHER, GUENTHER, BUCKEL, PETER
Format:	Patent
Sprache:	dan
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A creatine amidinohydrolase in which the alanine in position 109 of the amino-acid sequence of the wild-type enzyme is replaced by valine and, where appropriate, additionally one or more other amino acids of the wild-type enzyme are mutated is more stable than the wild-type enzyme and is therefore more suitable for reagents. It is prepared by bringing about the expression, by known genetic engineering methods in a suitable expression system, of a recombinant DNA which contains a creatine amidinohydrolase gene which contains the deoxyribonucleotide T in position 326 of the natural gene in place of C.