Fremstilling af koagulationsfaktor IX med forbedret glycosyleringsmønster

Fremstilling af koagulationsfaktor IX med forbedret glycosyleringsmønster

An improved system for large scale production of antibodies in cell culture is provided. In accordance with the present invention, cells expressing an antibody are grown in media that contain manganese at a concentration of between approximately 10 and 600 nM. The use of such a system allows product...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Koza, Stephan M, Lasko, Daniel R
Format: Patent
Sprache:dan
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:An improved system for large scale production of antibodies in cell culture is provided. In accordance with the present invention, cells expressing an antibody are grown in media that contain manganese at a concentration of between approximately 10 and 600 nM. The use of such a system allows production of an antibody with an increased glycosylation pattern and/or a glycosylation pattern that more accurately reflects the glycosylation pattern of the naturally occurring antibody. An antibody expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical compositions.