Fraktionering af proteinholdige blandinger

A primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an expanded bed adsorption column comprising an adsorbent, said adsorbe...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ANDERSEN, INGA VAARST, OLANDER, MORTEN AAE, LIHME, ALLAN OTTO FOG, HANSEN, MARIE BENDIX
Format: Patent
Sprache:dan
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Beschreibung
Zusammenfassung:A primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an expanded bed adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high-density non-porous core, surrounded by a porous material; the absorbent having a particle density of at least 1.5g/ml, wherein said high-density non-porous core has a density of at least 4 g/ml, and the degree of expansion (H/H0) of the adsorbent is in the range of 1.2-5, and wherein the flow rate of applying said protein-containing mixture is at least 8 cm/min; c) optionally washing the column; d) eluting at least one protein from the adsorbent.