Fremgangsmåder til at vurdere celler og cellekulturer
The present invention concerns a method of evaluating the composition of a cell culture, said method comprising: a) obtaining a plurality of cells from a cell culture; b) determining the average expression level of a fibroblast marker in the plurality of cells, said fibroblast marker being such that...
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| Zusammenfassung: | The present invention concerns a method of evaluating the composition of a cell culture, said method comprising:
a) obtaining a plurality of cells from a cell culture;
b) determining the average expression level of a fibroblast marker in the plurality of cells, said fibroblast marker being such that its normalized expression levels are lower in chondrocytes than in fibroblasts or synoviocytes; and
c) determining the composition of the culture based on the average expression level of the fibroblast marker,
wherein the average expression level below a predetermined threshold indicates that the cell culture comprises chondrocytes, and wherein said fibroblast marker is micro-fibrillar associated protein 5 (MFAP5). |
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