FREMGANGSMÅDE TIL FREMSTILLING AF INSULINKONJUGATER

FREMGANGSMÅDE TIL FREMSTILLING AF INSULINKONJUGATER

The invention claims a process for making an insulin-oligomer conjugate IN-105. IN-105 precursor having formula G-A-V-R-[B-Chain]-R-D-A-D-D-R-[A-Chain] is cloned and expressed in Pichia. The biosynthetic precursor is then conjugated with an activated oligomer. The IN-105 precursor-oligomer conjugate...

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Bibliographische Detailangaben
Hauptverfasser: GARG, MAYANK, SACHDEV, GOLDY, KHEDKAR, ANAND, DAVE, NITESH, HAZRA, PARTHA, GOEL, ANUJ, IYER, HARISH, SURYANARAYAN, SHRIKUMAR, KRISHNAN, GAUTAM, ROY, NITA, MANIKAM, GOVINDASAMY, MANJUNATH, H. S
Format: Patent
Sprache:dan
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Beschreibung
Zusammenfassung:The invention claims a process for making an insulin-oligomer conjugate IN-105. IN-105 precursor having formula G-A-V-R-[B-Chain]-R-D-A-D-D-R-[A-Chain] is cloned and expressed in Pichia. The biosynthetic precursor is then conjugated with an activated oligomer. The IN-105 precursor-oligomer conjugate is then treated with protease and purified to afford active insulin-oligomer conjugate of formula insulin-OC-CH2-CH2-(OCH2CH2)3-OCH3.