Fremgangsmåde til bestemmelse af DNA-methylering i blod- eller urinprøver

Aspects of the present invention relate to compositions and methods for providing DNA fragments from a remote sample. In particular aspects a remote sample comprising DNA is provided, DNA is isolated from the remote sample, and the isolated DNA is treated in a way which allows differentiation of met...

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Hauptverfasser: DEVOS, THEO, TETZNER, REIMO, SLEDZIEWSKI, ANDREW, BALLHAUSE, MATTHIAS, DIETRICH, DIMO, LOFTON-DAY, CATHY, SCHUSTER, MATTHIAS, LIEBENBERG, VOLKER, BERLIN, KURT, MAAS, JENNIFER, MODEL, FABIAN, LOGRASSO, JOE
Format: Patent
Sprache:dan
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Beschreibung
Zusammenfassung:Aspects of the present invention relate to compositions and methods for providing DNA fragments from a remote sample. In particular aspects a remote sample comprising DNA is provided, DNA is isolated from the remote sample, and the isolated DNA is treated in a way which allows differentiation of methylated and unmethylated cytosine. Additional, particular embodiments provide compositions and methods for methylation analysis of DNA derived from a remote sample. Other aspects provide for compositions and methods of whole genome amplification of bisulfite treated DNA.