In vitro isolering og opretholdelse af embryone stængelceller, (ES)-celler, samt fremgangsmåde til fremstilling af et non-humant kimært dyr

PCT No. PCT/AU89/00330 Sec. 371 Date May 31, 1990 Sec. 102(e) Date May 31, 1990 PCT Filed Aug. 3, 1989 PCT Pub. No. WO90/01541 PCT Pub. Date Feb. 22, 1990.The present invention relates generally to the use of leukaemia inhibitory factor (LIF) in the maintenance and derivation of embryonic stem (ES)...

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Hauptverfasser:	WILLIAMS, ROBERT LINDSAY, HILTON, DOUGLAS JAMES, GOUGH, NICHOLAS MARTIN
Format:	Patent
Sprache:	dan
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE
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Zusammenfassung:	PCT No. PCT/AU89/00330 Sec. 371 Date May 31, 1990 Sec. 102(e) Date May 31, 1990 PCT Filed Aug. 3, 1989 PCT Pub. No. WO90/01541 PCT Pub. Date Feb. 22, 1990.The present invention relates generally to the use of leukaemia inhibitory factor (LIF) in the maintenance and derivation of embryonic stem (ES) cells in culture. The ES cells are maintained and/or derived from animal embryos by culturing said cells or embryos in a culture medium containing an effective amount of LIF for a time and under conditions sufficient to maintain and/or derive said ES cells. The ES cells may be passaged in LIF and used to make chimaeric animals.