Rekombinant plasmid og DNA-isolater omfattende et DNA-segment som koder for et enzym med 2,5-DKG-reductase-aktivitet, rekombinant ekspressionsvektor som kan frembringe ekspression af en sådan DNA-sekvens, mikrobielle værtceller hvori

A protein having 2,5-diketogluconic acid (2,5-DKG) reductase activity is produced by recombinant host cells. This can be used to convert 2,5-DKG stereoselectively into 2-keto-L-gluonic acid (2-KLG) which is a useful intermediate in the production of ascorbic acid (vitamin C).

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Bibliographische Detailangaben
Hauptverfasser:	LAZARUS, ROBERT ALAN, ESTELL, DAVID AARON, RASTETTER, WILLIAM HARRY, MILLER, JEFFREY VEACH, LIGHT, DAVID RICHARD
Format:	Patent
Sprache:	dan
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HETEROCYCLIC COMPOUNDS HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A protein having 2,5-diketogluconic acid (2,5-DKG) reductase activity is produced by recombinant host cells. This can be used to convert 2,5-DKG stereoselectively into 2-keto-L-gluonic acid (2-KLG) which is a useful intermediate in the production of ascorbic acid (vitamin C).